BIOC 662

Macromolecular Interactions

This is a practical course that coordinates lectures with experience in the Macromolecular Interactions Facility. Lectures introduce methods for monitoring interactions of macromolecules with other molecules. Labs offer students hands-on experience with major techniques available in the Facility.

Instructor: Ashutosh Tripathy; 962-2362 or Ashutosh_Tripathy@med.unc.edu

Meeting Dates/Times and Locations: 3/23/15-4/24/15; Lectures MW 11-11:50AM in 3007 GMB; Labs TBA in MacInFac. Registration limited to 16 students. Cross listed as Chem 735.

Introduction to Binding and to Methods in the MacInFac

Binding
Introduction to binding
Direct measurements
Indirect measurements

Analytical Ultracentrifugation
Introduction to analytical ultracentrifugation
Sedimentation velocity
Sedimentation equilibrium

Surface Plasmon Resonance (SPR)
Principles
Kinetics of macromolecular interactions
Analysis of macromolecular binding equilibria

Biological microcalorimetry
Principles
Differential scanning calorimetry and macromolecular domain structure
Isothermal titration microcalorimetry to monitor macromolecular binding

Static and Dynamic light scattering and determination of molar mass, RMS radius and hydrodynamic radius of macromolecules