BIOC 663A and 663B

Macromolecular NMR: Theory &Practice

Instructors:  Sharon Campbell (Course Coordinator), 966-7139; Qi Zhang,; and Greg Young.

Meeting Dates/Times and Locations: One (lecture) or two (lecture plus lab) credit hours; Lectures: 2/11/15-3/20/15 MWF 11-11:50AM, 3007 Genetics Medicine; labs: 3 hours per week in Marsico Hall (time and day TBA)]. Cross listed as Chem 734.

Pulse NMR: Basic principles and methods

Laboratory: Simple experiments in D2O

Spin relaxation: The theory and measurement of T1 and T2

Laboratory: Data management and treatment

Spin coupling: Scalar (through-bond) coupling

Laboratory: A simple protein in water

Dipolar (through-space) coupling (the NOE) and polarization transfer

Laboratory: 1D NOE

2D homonuclear NMR: Principles and methods

Laboratory: 2D COSY and NOESY in D2O and H2O