BIOC 666

X-ray Crystallography of Macromolecules

Instructor: Hengming Ke; 919-966-2244, hke@med.unc.edu

Meeting Dates/Times and Locations: 1/7/15-2/9/15; 9:00-9:50, MWF; 3007 Genetics Medicine

Crystallization of macromolecules

Crystal symmetry and space group

Nature of X-rays and principles of diffraction of a single crystal

Methods to phase a macromolecular structure: Patterson function, multiple isomorphous replacement, multiwavelength anomalous diffraction, and molecular replacement

Phase improvement

Preparation of heavy atom derivatives

Interpretation of electron density and model building of a protein structure

Refinement of a raw macromolecular structure