BIOC 652

Macromolecular Structure and Dynamics

Instructor: Nikolay Dokholyan; 919-843-2513,

Meeting Dates/Times and Locations: 1/7/15-2/09/15; MWF 11:00 AM; 3007 GMB Registration limited to 12 except with permission Course Director. (Recitation will meet on Tuesdays and Thursdays, Time and location will be determined).

Conformational thermodynamics of macromolecules: Understanding polymer

Thermodynamics of protein folding
Protein folding
Two-state protein folding
Differential Scanning Calorimetry
Effect of mutations on folding
Cold denaturation
Solvent-induced denaturation
Protein surface area
Protein stability
Helix-coil transition

Kinetics of chemical reactions
Chemical reactions: Terminology; Treatment; Equilibrium
First-order chemical reactions
Second-order chemical reactions
Transition state theory
Enzyme kinetics
Diffusion-limited reactions
Relaxation to equilibrium
Consecutive reactions
Diffusion in the external field.

Protein folding kinetics
Two-state folding kinetics
Stopped-Flow Fluorescence
Chevron plot
Tanford β-value
Three-state folding kinetics, nucleation
Timescales of protein motion
H/D exchange
Nucleation mechanism of protein folding

Stochastic processes
Markov Process
Random walk: Poisson Process
Brownian Motion: Langevin Equation
Fluctuation-Dissipation Theorem
Fokker-Plack Equation