BIOC 652 Macromolecular Structure and Dynamics
Instructor: Brian Kuhlman; 919-843-0188, bkuhlman@email.unc.edu
Meeting Dates/Times and Locations: November / Early December; MWF 11:00 AM; 3007 GMB Registration limited to 12 except with permission Course Director. (Recitation TBD).
Conformational thermodynamics of macromolecules: Understanding polymer
Thermodynamics of protein folding
- Protein folding
- Two-state protein folding
- Differential Scanning Calorimetry
- Effect of mutations on folding
- Cold denaturation
- Solvent-induced denaturation
- Protein surface area
- Protein stability
- Helix-coil transition
Kinetics of chemical reactions
- Chemical reactions: terminology, treatment, equilibrium
- First-order chemical reactions
- Second-order chemical reactions
- Transition state theory
- Enzyme kinetics
- Diffusion-limited reactions
- Relaxation to equilibrium
- Consecutive reactions
- Diffusion in the external field
Protein folding kinetics
- Two-state folding kinetics
- Stopped-flow fluorescence
- Chevron plot
- Tanford β-value
- Three-state folding kinetics, nucleation
- Timescales of protein motion
- H/D exchange
- Nucleation mechanism of protein folding
Stochastic processes
- Markov Process
- Random walk: Poisson Process
- Brownian Motion: Langevin Equation
- Fluctuation-Dissipation Theorem
- Fokker-Plack Equation