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Instructors: Dorothy Erie (

Last third of the spring semester – offered in odd numbered years; module discussion course

Principles of fluorescence spectroscopy followed by a survey of methods and applications in fluorescence microscopy, including:

  • Single molecule microscopy
  • Super-resolution microscopy
  • Total internal reflection fluorescence microscopy
  • Fluorescence polarization microscopy
  • Fluorescence resonance energy transfer microscopy
  • Fluorescence lifetime microscopy
  • Fluorescence recovery after photobleaching
  • Fluorescence correlation spectroscopy
  • Single fluorescence particle tracking