BIOC 673: Proteomics , Protein Identification, and Characterization by Mass Spectrometry

Instructor: Xian Chen (xianc@email.unc.edu)

Dates: 2/20/2024-3/26/2024

Times: TTH 12:30-1:45PM

Location: TBD

Proteomics or the study of protein compliments of a given genome is emerging as the mainstream of systems biology. This course will start from the introduction about how mass spectrometry (MS) operates for protein analysis. The unique strengths of modern MS in analyzing protein groups in complexes or even in cells/tissue in situ will be presented alongside with a variety of the associated cutting-edge MS technology. We will then work through an array of both conventional and latest MS-based proteomic techniques/methods in identifications/profiling of differential expressing proteins, post-translational modifications (phosphorylation, acetylation, ubquitination, methylation, etc), protein-protein interactions, and protein secretion –  the key components involved in any disease pathway or mechanism. Finally, applications of the state of the art technology of MS in biomarker or drug target discovery will be illustrated.