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Instructors:  Sharon Campbell (Course Coordinator) campbesl@med.unc.edu, Stu Parnham, (919) 966-7139

Meeting Dates: 1.0 (lecture) or 2.0 (lecture plus lab) credit hours; offered in odd numbered years

Times: MWF 11:15AM-12:05PM

Locations: GMB 3007

*Labs – 3 hours per week in Marsico Hall (time and day TBA). Cross listed as Chem 734.

UNC Biomolecular NMR Laboratory

Topics:

  • Pulse NMR: Basic principles and methods
  • Laboratory: Simple experiments in D2O
  • Spin relaxation: The theory and measurement of T1 and T2
  • Laboratory: Data management and treatment
  • Spin coupling: Scalar (through-bond) coupling
  • Laboratory: A simple protein in water
  • Dipolar (through-space) coupling (the NOE) and polarization transfer
  • Laboratory: 1D NOE
  • 2D homonuclear NMR: Principles and methods
  • Laboratory: 2D COSY and NOESY in D2O and H2O