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Instructor: Hengming Ke; 919-966-2244, hke@med.unc.edu

Meeting Dates: 01/10/2022 – 02/11/2022

Times: MWF 9:05 – 9:55AM

Location: GMB 3007

Topics:

  • Crystallization of macromolecules
  • Crystal symmetry and space group
  • Nature of X-rays and principles of diffraction of a single crystal
  • Methods to phase a macromolecular structure: Patterson function, multiple isomorphous replacement, multiwavelength anomalous diffraction, and molecular replacement
  • Phase improvement
  • Preparation of heavy atom derivatives
  • Interpretation of electron density and model building of a protein structure
  • Refinement of a raw macromolecular structure