BIOC 666 X-ray crystallography of Macromolecules

Instructor: Hengming Ke; 919-966-2244, hke@med.unc.edu

Meeting Dates: 01/10/2024 – 02/14/2024

Times: MWF 9:00AM – 9:50AM

Location: GMB 3007

BIOC 666 Syllabus

Topics:

• Crystallization of macromolecules
• Crystal symmetry and space group
• Nature of X-rays and principles of diffraction of a single crystal
• Methods to phase a macromolecular structure: Patterson function, multiple isomorphous replacement, multiwavelength anomalous diffraction, and molecular replacement
• Phase improvement
• Preparation of heavy atom derivatives
• Interpretation of electron density and model building of a protein structure
• Refinement of a raw macromolecular structure