BIOC 666 X-ray crystallography of Macromolecules

Instructor: Hengming Ke (hke@med.unc.edu)

Meeting Dates: 01/08/2025 – 02/12/2025

Times: MWF 9:00AM – 9:50AM

Location: TBD

BIOC 666 Syllabus

Topics:

• Crystallization of macromolecules
• Crystal symmetry and space group
• Nature of X-rays and principles of diffraction of a single crystal
• Methods to phase a macromolecular structure: Patterson function, multiple isomorphous replacement, multiwavelength anomalous diffraction, and molecular replacement
• Phase improvement
• Preparation of heavy atom derivatives
• Interpretation of electron density and model building of a protein structure
• Refinement of a raw macromolecular structure