BIOC 666 X-ray crystallography of Macromolecules
Instructor: Hengming Ke (hke@med.unc.edu)
See Connect Carolina for current dates, meeting pattern, and location.
Topics:
- Crystallization of macromolecules
- Crystal symmetry and space group
- Nature of X-rays and principles of diffraction of a single crystal
- Methods to phase a macromolecular structure: Patterson function, multiple isomorphous replacement, multiwavelength anomalous diffraction, and molecular replacement
- Phase improvement
- Preparation of heavy atom derivatives
- Interpretation of electron density and model building of a protein structure
- Refinement of a raw macromolecular structure