BIOC 667 Macromolecular Crystallographic Methods
Instructors: Nathan Nicely (nnicely@email.unc.edu)
Meeting Dates: 01/11/2024 – 02/15/2024
Times: TTh 2:00-3:15PM
Location: Medical Research Building B
*Note permission of course director required
This is a practical workshop for students of protein crystallography. The course is hybrid lecture and laboratory. Attendance is absolutely essential especially for the laboratory components. Students will crystallize proteins then collect and process x-ray diffraction data. There is no specific text required, but several options for further study will be recommended.
Outline
- Crystal architecture
- Crystallization of biomolecules (practical)
- Preparing crystals for x-ray diffraction data acquisition (practical)
- Strategizing and collecting x-ray diffraction data (practical)
- Bragg’s law and Ewald’s construction for visualizing diffraction
- Data reduction, statistical analyses, and other indicators of quality (practical)