BIOC 667 Macromolecular Crystallographic Methods
Instructors: Nathan Nicely (nnicely@email.unc.edu)
Meeting Dates: 03/23/2023 – 04/25/2023
Times: TTh 12:30-1:45PM
Location: Medical Research Building B
*Note 1 credit Prerequisite BIOC 666 or permission of course director.
This is a practical workshop for serious students of protein crystallography. There will be a laboratory component that will be arranged to accommodate the schedules of students and faculty. In the laboratories, students will crystallize protein, collect and process their data, determine phases and refine their structures. There is no specific text required, but Jan Drenth “Principles of Protein X-ray Crystallography” is recommended.
Part I
- Data Collection (Collins)
- Bragg’s law and Ewald’s construction for visualizing diffraction
- Detectors
- Symmetry
- Corrections
- Integration
- Data analysis
Part II
- Phase determination (Redinbo)
- MR
- MIR
- AS
- MAD
- Direct methods
Part III
- Refinement (Collins)
- Computational
- Targets
- Techniques
- Manual intervention
- Computational
Laboratory portion
- Crystallize lysozyme
- Collect data set (teams)
- Data reduction
- Molecular replacement
- Introduction to “O”
RESOURCES
Carolina Biophysics Symposium
Biophysics Summer Course
Biophysical Society Annual Meeting